Hesa-A Effects on Cell Cycle Signaling in Esophageal Carcinoma Cell Line

Nasser Ahmadian, Roghiyeh Pashaei-Asl, Nasser Samadi, Mohammad Rahmati-yamchi, Mohammad-Reza Rashidi, Masomeh Ahmadian, Moosa Esmaeili, Faezeh Salamat, Sima Besharat, Hamid Reza Joshaghani

Abstract


BACKGROUND

Hesa-A is a natural compound with anticancer properties. The exact mechanism of its action in esophageal cancer is not clear, yet. The aim of this study was to evaluate the cell toxicity effect of Hesa-A on the esophageal carcinoma cell lines, KYSE-30, and cell cycle genes expression.

METHODS

In this study, we tested cell toxicity with MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay and flow cytometry to evaluate the cell cycle arrest. Real time polymerase chain reaction was used to assess the expression of P53, P16, P21, cyclin D1, and cyclin B1 genes.

RESULTS

Our results showed that Hesa-A is effective in the expression of cell cycling check point proteins. Hesa-A induced an arrest in G2 phase of esophageal cell cycle. The levels of P53 (>13 times), P21 (>21 times), P16, cyclin B1, and cyclin D1 genes were increased 48 hours after Hesa-A treatment.

CONCLUSIONS

P21 and P16 expression were the potential mechanisms for G2 arrest of KYSE-30 esophageal cancer cell line by Hesa-A.


Keywords


Hesa-A; Esophageal cancer; Flow cytometry; Real Time PCR; P53 gene; P16 gene; P21 gene; Cyclin D1 gene; Cyclin B1 gene

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