Introducing New Potential Biomarkers for Celiac Disease among the Genes Extracted from General Databases

Ensieh Khalkhal, Mostafa Rezaei-Tavirani, Nastaran Asri, Fatemeh Nobakht, Somayeh Jahani-Sherafat, Mohammad Hosain Haidari, Mohammad Rostami-Nejad



Inflammatory cytokines play roles in the pathogenesis of celiac disease. To introduce new diagnostic markers in patients with celiac disease for easy, fast, low cost, and non-invasive diagnosis, we evaluated the peripheral blood expression levels of Interleukin 15 (IL15), Interleukin 17A (IL17A), Interleukin23A (IL23A), granzyme B (GZMB), T-Box Transcription Factor 21 (TBX21), and TNF Alpha Induced Protein 3 (TNFAIP3) of patients compared with the healthy controls, which were extracted from public databases organized in a protein-protein interaction network, in this group.


 Peripheral blood mononuclear cells were collected from 30 patients with celiac disease and 30 healthy subjects. Total RNA was extracted, and mRNA expression levels of targeted genes were investigated by the quantitative real-time Polymerase chain reaction (PCR) method. SPSS software was used for statistical analysis. Receiver operating characteristic curve (ROC curve) analysis was performed to characterize the diagnostic ability of the studied genes.


The expression of IL15, IL17A, IL23A, GZMB, TBX21, and TNFAIP3 genes in peripheral blood mononuclear cells of patients with celiac disease showed a significant increase compared with the control group. Among them, TNFAIP3, IL23A, and GZMB have better resolution and diagnostic value in differentiating patients with celiac disease from healthy controls.


 Our results suggest that TNFAIP3, IL23A, and GZMB could be useful and sensible markers in differentiating patients with celiac disease from healthy controls. However, the diagnostic relevance of other genes recognized by pathway analysis needs to be further investigated.


Autoimmune diseases; Blood; Celiac disease; Inflammation; Polymerase chain reaction

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